Composite

Part:BBa_K3287001:Design

Designed by: Diego Vinatea Samperio   Group: iGEM19_UPNAvarra_Spain   (2019-10-08)


Nit_Yellow


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We do not have any special consideration


Source

The source of the part including references: The nitrate sensitive promoter was firstly used by BCCS-Bristol team in iGEM 2010. After that, other teams have studied and characterized this part. It is the promoter of the Escherichia coli yeaR/yoaG operon, described by Lin et al., 2007. We placed the amilGFP yellow protein behind the PyeaR promoter. This chromoprotein was design by the Uppsala-Sweden team in iGEM 2011, exhibiting strong color when expressed.


References